Composite

Part:BBa_K4695111:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


estJ6-docscaB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part consists of:

  • EstJ6 coding sequence (BBa_K4695011)
  • DT4_TEV linker (BBa_J435395)
  • DocScaB coding sequence (BBa_K4695041)
  • C-terminal T5E_6H HisTag linker (BBa_J435369)


Sequence is designed in a way that allows user to swap enzymatic and dockerin domains with their counterparts.
FLANKED 5' fusion site: AATG, 3' fusion site: GCTT.
Source sequences were optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/


Source

EstJ6:
Soil metagenomic library
GenBank: https://www.ncbi.nlm.nih.gov/nuccore/MK037455.1/
Article: https://doi.org/10.1016/j.ecoenv.2019.110148

DocScaB:
Organism: Acetivibrio cellulolyticus
Structure: https://www.rcsb.org/structure/4UYQ

Registry of standard biological parts

References